网络棋牌

讨论分析酶底物法与酶底物法封膜机应用的优势与不足

- 2019-05-21-

网络棋牌    MUQIANWOGUODUISHENGHUOYINYONGSHUIJINXINGWEISHENGXUEPINGJIA ,JIANCEDEWEISHENGWUZHIBIAOZHUYAOYOUDACHANGJUNQUN、NAIREDACHANGJUNQUN、DACHANGAIXIJUN ,ZHEILEIZHIBIAOSHIFENBIANWURANDEZHISHIJUN。JINGDIANDECHUANTONGFANGFAWEIDUOGUANFAJIAOFA ,QIJIANCEZHOUQIJIAOZHANG ,XU 2 ~5 d,YANGXINGJIEGUOXUYANZHENGSHIYAN ,SHIDESHIYANCAOZUOBUZHOUJIAOWEIFANSUO ,BUNENGDUISHENGHUOYINYONGSHUIDEWEISHENGXUEZHUANGKUANGZUOCHUKUAISUPINGJIA ,TEBIESHIZAIKANGZHENJIUZAITESHUSHIQI ,RUNENGSHIXIANWEISHENGWUZHIBIAODESHISHIJIANCE ,JIANGJIDADIZHICHIKANGZHENJIUZAIGONGZUODESHUNLISHISHI ,YOULIBAOZHANGGUANGDAZAIQURENMINQUNZHONGSHENGHUOYINYONGSHUIDEGONGSHUIANQUAN。YINCI , MEIDIWUFAZUOWEIYIZHONGKUAIJIANFANGFA ,QIKUAISUJIANBIANDETEDIANKEYIJIAOHAODEMIBUCHUANTONGFANGFADEBUZU。ERMEIDIWUFAFENGMOJIYESHIQIZHONGYIXIANGHENZHONGYAODEJIANCEYIQISHEBEI。

    MEIDIWUFA(enzyme substrate technique)DEYUANLISHI :LIYONGDACHANGJUNQUNXIJUNNENGCHANSHENGβ2BANRUTANGGANMEI (β2D2galactosidase)FENJIE ON 2PG (O rtho2 nitrophenyl2β2D2galactopyranoside)SHIPEIYANGYECHENGHUANGSE ,JIANCESHUIYANGZHONGSHIFOUHANYOUDACHANGJUNQUN。LIYONGDACHANGAIXIJUNCHANSHENG β2PUTAO TANG QUAN SUAN MEI (β2glucuronidase )FENJIEMUG( 42 methyl2umbelliferyl2β2D2glucuronide)SHIPEIYANGYEZAIBOZHANG 366 nmZIWAIGUANGXIACHANSHENGLANSEYINGGUANG,JIANCESHUIYANGZHONGSHIFOUHANYOUDACHANGAIXIJUN。MEIDIWUFAYOUSHANGPINHUASHIJIGONGYING ,SHIYANCAOZUOJIANBIAN ,WUXUQUERENSHIYAN ,KETONGSHIJIANCESHUIYANGZHONGDACHANGJUNQUNHEDACHANGAIXIJUN。RUGUOGAIBIANPEIYANGWENDU ,HAIKEJIANCENAIREDACHANGJUNQUN。GAIFANGFAJIANCEZHOUQIDUAN,18~24hJIKEBAOGAOZUIZHONGJIEGUO。KEJIANGAIFANGFAZUIDADETEDIANSHIJIESHENGLESHIJIANHERENLI。

酶底物法应用的优势:
准确可信
网络棋牌    使用MPN法进行定量检测,可直接检测100毫升水扬中0-2419MPN目标细菌,精确检出100ml水样中单个的活性大肠杆菌群和大肠埃希氏菌,假阴性低,每个单位试剂可抑制上百万个异养细菌,假阴性低,无需验证,准确性高于滤膜法及多管发酵法,符合中国国家标准GB/T5750.12-2006《生活饮用水检验标准》。

简单快捷
    检测时间不超过24小时,无需验证试验,简单培训就能轻松使用,手工操作少于一分钟,不会因为过滤阻塞(滤膜法)或异养细胞的干扰(多管发酵法)而产生数据误差,无需玻璃器皿清洗及菌落计数,减轻实验人员工作量,适用于大量样品的批量快速检测。

MEIDIWUFADEZHUYAOQUEDIAN

    PEIYANGJICHENGFENFUZA ,SHIYANSHIPEIZHIKUNNAN ,MUQIANZHIYOUJINKOUSHIJIKEGONGXUANZE ,SHIDEJIANCECHENGBENDAWEITIGAO。RUGUONENGSHIXIANSHIJIDEGUOCHANHUA ,XIANGXINQIYINGYONGHUIDEDAOJIDADITUIGUANG。DUIYUWURANBIJIAOYANZHONGDESHENGHUOYINYONGSHUI ,MEIDIWUFADEDINGLIANGJIANCENENGLIBURUDUOGUANFAJIAOFA。YUDAOZHEILEIQINGKUANGSHIRUXUYINGYONGMEIDIWUFA ,ZEZHINENGXIANJIANGSHUIYANGSHIDANGXISHIHOUZAIJINXINGJIANCE。

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